This Update came a little bit late cuz time issues but here is it :) Last week i had solution issues and the plants go very fast in bad leaf color. The Solution got too warm in the tanks and the water got a bad smell too. I changed the water and made again the 3rd week nutrition and turned up the fan to get a few degrees colder it worked very well. Roots are so far doing nice and have no burns or rotten parts on nice and white. Then i installed the second light in. The week before i give them 400 W MH and 100 W LED. Now we have 2x 600W and 100 W LED each one turned down to 250 W and the going good. But i had only 1 bulb m MH and so im now have a mixed light end of this week i give them 1 tsp Mineral Magic over the stem to get them stronger Mostly of them are Topped and have not bad mooding on the growing stage of the plants. I hope on get a few Tips on what we can do better on the System Best Regards :) and Good Groow @all

🌱 Week 4 Veg: Thriving Despite the Chill 🌟 As we close out Week 4 of veg, I couldn’t be more impressed with how resilient and strong the Zamnesia Runtz are growing! Despite the freezing cold temperatures outside—evident in some snapshots of my frosty mint plants in the veggie garden—these beauties are thriving in their cozy indoor setup. The Aptus Holland Nutrient Spray: A Deep Dive This week, I treated the Runtz to a refreshing shower with Aptus Holland’s nutrient spray, a game-changer in plant health and vigor. Let’s break it down: • What Is It? Aptus nutrient sprays are foliar feeds designed to deliver essential nutrients directly to the leaves. They provide immediate uptake, bypassing the roots and giving the plant a quick boost in vitality. • Why Use It? Foliar feeding is especially beneficial during veg because it enhances nutrient absorption and promotes lush, healthy foliage. It’s also a fantastic way to supplement nutrients if the roots are growing but not yet absorbing nutrients at peak efficiency. • What Does It Do? Aptus sprays improve plant metabolism, strengthen cellular structure, and boost resistance to environmental stresses—perfect for battling those colder temps! • How I Applied It I used a fine mist to ensure even coverage across all leaves, aiming for the undersides where stomata are most active. Afterward, the plants looked vibrant and energized, ready to keep pushing those gorgeous leaves. A New Partnership: Grove Bags and TerpLoc Technology I’m thrilled to announce a new partnership with Grove Bags! Known for their TerpLoc technology, these bags are a true revolution in cannabis storage and curing. • Why Grove Bags? Grove Bags are designed to create the perfect microclimate for cannabis. Their TerpLoc technology helps preserve terpenes—the essential oils responsible for the flavor, aroma, and effects of cannabis—by locking them in while allowing oxygen and moisture to escape. • How They Work TerpLoc bags combine several unique layers: • Moisture Control: Maintains ideal humidity levels to prevent over-drying or mold. • Oxygen Barrier: Blocks excessive oxygen exposure, which can degrade terpenes. • UV Protection: Shields the buds from harmful light that can alter their chemical composition. • Why This Matters For anyone who’s spent months cultivating a crop, ensuring the harvest cures and stores properly is the final crucial step. Grove Bags will play an essential role in preserving the quality and flavor of this Runtz grow, keeping the terps intact for that unforgettable smoke experience. A big thank you to Grove Bags for joining this journey. I can’t wait to showcase their role in the final stages of this grow! Photo Magic: Nikon P900 & Sony A6000 Capturing the Runtz in all their glory is part of the journey, and this week’s shots were nothing short of stunning. Using both the Nikon P900 and Sony A6000 allowed for crisp, detailed photos that highlight the beauty of these young plants. From their vibrant green tones to the intricate structure of their leaves, these images tell the story of strength and potential. Week 4 Veg: What It Means Week 4 in veg is a critical time in a grower’s journey. By now, the plants have developed a strong root system and are focusing on building structure. This is when: • Leaf Development Peaks: Bigger leaves mean more photosynthesis and energy production. • Growth Accelerates: Plants use this time to set the foundation for flowering, with nodes spacing out and stems thickening. • Preparation for Transplanting: Root systems are expanding, making it the perfect time to plan for a move into larger pots, as we’ll be doing next week! Looking Ahead Next week, the Runtz will graduate to larger pots—not their final AutoPot homes yet, but an essential step for continued growth. This transition will help the roots spread further, setting the stage for a thriving flowering phase. Community & Gratitude A huge thank you, as always, to my sponsors and supporters: • Aptus Holland • Pro-Mix Soil • TrolMaster Ecosystem • ThinkGrow LED • Future of Grow LED • CannaKan Germination Station • Zamnesia and Plagron for making this Eternity Cup journey so special • Ziggi Papers • And now, our newest partner: Grove Bags To the amazing Grow Diaries community, you continue to inspire me daily. I’ve been blown away by the incredible work and creativity I’ve seen in other diaries—it’s motivating and humbling all at once. Good luck to everyone; let’s make this journey one to remember! 💚 Final Thoughts From the frosty outdoors to the warmth of my grow room, these Runtz are proving their resilience and promise. Each week, I’m reminded of why I fell in love with this strain, and the excitement for what’s to come keeps growing. Stay tuned for Week 5, where we’ll delve into the transplant process, nutrient updates, and more. Let’s keep learning, growing, and sharing the magic together! Genetics - Runtz https://www.zamnesia.com/6000-zamnesia-seeds-runtz-feminized.html Nutrients - Plagron https://plagron.com/en/hobby - Aptus Holland https://aptus-holland.com/ Controls - Trol MAster https://www.trolmaster.eu/ LED - https://www.futureofgrow.com/en LED - https://www.thinkgrowled.com Soil - https://www.promixgardening.com/en Germination - Cannakan https://cannakan.com/?srsltid=AfmBOopXr-inLXajXu3QFgKXCXXos4F1oEvScjMKIB5MR5dk8-GJ-F49 DOGDOCTOR 15% off Smoking Papers - https://ziggioriginal.com/ Terpene saver - https://grovebags.com/ As always thank you all for stopping by, for the love and for it all , this journey of mine wold just not be the same without you guys, the love and support is very much appreciloved and i fell honored with you all in my life With true love comes happiness Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so As always, this is shared for educational purposes, aiming to spread understanding and appreciation for this plant. The journey with nature is one of discovery, creativity, and respect. Let’s celebrate it responsibly and continue to learn and grow together! Growers Love To you All 💚 #EternityGrowCup #RuntzHunt #GrowersLove #CannabisCommunity #AptusHolland #ProMixSoil #TrolMaster #Zamnesia #Plagron #ZiggiPapers #Grovebags

4ª settimana di fioritura alla CAMPACAVALLO in collaborazione con TENT-X 🤖⚡🍋 Abbiamo ripulito un po' la parte bassa della bella CRITICAL LEMON ⚡🍋 con la app LUX misuriamo circa 60000 lux🌞 Inizia a bere come un cammello la ragazza 🐫... Circa 1 litro al giorno 🍺

🧡 ACAPULCO GOLD 🧡 This is becoming a very interesting pheno! She has developed 3 tops, and growing really well, nice and healthy, and is looking so far 👌 I have a full tent, so I'm considering letting the plants in this grow, grow up as I've got plenty of head space but very little horizontal space. This one is a Sativa, and so I am preparing for a tall plant. This was what I did this week: 23.6.25: I watered with 2L of dechlorinated water PH'd to 6.6 and containing no nutrients. PH: 6.6 PPM: 307 26.6.25 I gave 2l of dechlorinated water PH'd to 6.2 with no nutrients; PPM: 333 PH: 6.4 27.6.25: I top dressed the plant today, I watered yesterday. Top dress: ♡ 2 cups Worm Castings ♡ 1 TBSP Ecothrive Life Cycle ♡ 1/2 TBSP Ecothrive Charge ♡ 1 TBSP Cinnamon 29.6.25: I watered with 1L of dechlorinated water PH'd to 6.0-6.2 and containing the following nutrients per 5 litres; ♡ 1 Tsp Cal-Mag by Greenleaf Nutrients PH: 6.0-6.2 PPM: 1300 Thanks for checking out my diary 💚✌️🍃😊🌱

16 Pflanzen mit der Hand schneiden is schon ne ordentliche Arbeit, in zwei Wochen kommen die nächsten 16 🙈

Was supposed to update my diaries yesterday but literally forgot lol #edibles but they’re flowering nicely and stretching as they go! They’re truly loving life under the ts1000 can’t wait to see what the next week brings!

Week 10: 20/4 light schedule, 150 mp water per plant 2x, no fertilization anymore. Day 65: Next week Tueasday/beggining week 11/ we need to harvest because they will come from the council to check the house😂 Well, the previous one finished in 69 days, but I think they won't be properly ready by next week. I tried it and made me high anyways. I would give at least 2 weeks more to be done. Tomorrow I will check the trichomes with magnifying glass. They are beautiful stinky girls. Flowers are getting thicker and pistils are getting brownish. Day 66: I checked the trichomes with magnifying glass and it will be ready by next Tuesday. 7 days left with this day. They are beautiful just check in the video (Day 66). Day 67: It is crazy the last 3 days was so hot here in London. Today was 38 degrees!!!!!! Poor plants even with ventilation it goes up to 30-31 degrees. Well, it is alright because direct ventilation goes on so they won't be cooked. 😓😛 Day 70: It is the last day when they receive water and they just get once and half of the daily intake. 2 days before harvest I will not water them. Harvest day is on Tuesday 30th of July when they are 72 days old. I have checked the trichomes all good they are matured nicely cloudy so now it is very strong. They are very stinky girls 😋 Day 71: No more water for them..... Tomorrow is harvest day!!!!!😋

Wieder 2Wochen rum, pro Woche ca 10cm dazu gekommen. Mängel zu erkennen, CalMag gekauft ActiVera wird folgen.

Plante are recovering slowly I’m really happy with new nutrients plant are looking better I should have waited for total recover before flowering stage anyway nice buds are getting bigger

This week has been good new children just been born so keep humidity up and give the babies time and love

Girls are developing well, i have a minor issue but is making me uncomfortable, a bug for the third time something that by research seams to be a lace bug, basically is like little drops of black oil in the leafs this develops in to a little static larvae then it becomes black, is tiny. And apparently does nothing on the surface to the plant, but man this bastards are imortal, I’m in the point of dropping a nuke or using a flame tower in the grow room. I’m deep in to flower... Besides that fir the third time, all good...

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

During week 3 all plants are growing well inside the Growfor.Life-Closet and have a healthy green color. The latest addition: Double Dutch plant #3 (from Serious Seeds) is behind the rest, but also doing well. On Day 18 the biggest three plants, Double Dutch plant #1 and #2 and OG-Kush have gotten so big that a first round of defoliation was necessary. I do this in order to avoid big leaves touching each other and causing dampness to condensate between the leaves, which in turn can attract fungus and mold. Therefore i take off all the big fan leaves starting from the bottom of the plant and only leave the top two fan leaves on the plant. Now the air can flow freely through the canopy again and no leaves are touching and no dampness can condensate. The plants are still getting no extra nutrients, since the PLAGRON Grow-Mix I use should still have plenty in it. I only use RHIZOTONIC from CANNA one time in order to enhance the root-production. Now at this stage you already can clearly see differences between Double Dutch (from Serious Seeds) and OG-Kush (from Humboldt Seeds). The leaf shape is different (smaller and more pronounced fingers on the leaves of OG-Kush) and also the growth pattern (more like Indica in Double Dutch). On Day 19 I decide to do some LST (=low stress training) in order to train the 3 biggest plants into a bushier form. This is necessary, because the Growfor.Life-Closet is limited in height and I want the plants to fill out the whole area well with budding sites. I use stiff insulated copper-wire (standard electrical wire, pieces of appr. 30 cm length) and bend small arches, which I then carefully place over the top-part of a side-branch and push the open ends of the wire downwards into the soil so it holds the branch in place, while its bent outwards away from the main stem. This way I 'fan-out' the lowest 4 side-branches, so they are growing upwards at places where the light can reach them better. (see in detail in video) All plants respond well to the LST and turn their leaves and tips of the side-branches towards the light again until the next day. At day 19 the plants are now almost 20 cm tall, therefore I also decide to switch to 12/12 hours of light/darkness now in order to induce flowering. I change the program of the Growfor.Life-Closet from the standard-grow-mode (=18 hours of light per day) to the custom-flowering-program (12/12 hours of light, where I can choose the time when the lamps turn on). Now the plants will grow slower until they start to flower. I LOVE THE Growfor.Life-Closet, its sooooooooooooo BEAUTIFUL and soooooooooooooooo SILENT, a landmark technical development in my opinion! This is growing made stealthy and effortless, GROWING OF THE 21st CENTURY!

👉Alrighty Then👈 👉Apple And Banana 👈 week 4 of flower So we had some real nice growth this , had to do a little leaf management👈 flower mode activated...... Everything is looking good 👍I'm using well water which is hard so I'm having a few issue but I'm on it .... So ive up'd the nutrients to accommodate pre flower ..... Soil by Promix Nutrients by Cronks Lights by MarsHydro.ca FC4800 X UR45 X Adlite Deep Red And Blue Tent 4x4x6.5 & Equipment by MarsHydro.ca High reflectivity inner mylar Thick Oxford fabric Smooth heavy duty zipper Sturdy metal frame Zipper blackout cloth Good anti light leakage performance The 6in Inline kicks ass moves alot of air ●Blue Light Effect: Blue light shortens internodes, resulting in shorter, stronger plants, ideal for supporting fruit development later. During vegetative growth, blue light promotes lateral branch development effectively. Well this should be fun 🙃 Thanks to all my growmies out there for stopping by its much appreciated 👈 👉Happy Growing👈 Well this should be fun 🙃 Thanks to all my growmies out there for stopping by its much appreciated 👈 👉Happy Growing👈

Today, on day 36, the plants have noticeably grown, with heights now ranging from approximately 45–55 cm. We increased the nutrient concentration to 650 ppm, maintaining daytime room temperature at 26°C and nighttime at 22°C, CO2 concentration at around 600 ppm, and humidity at 65%. The light schedule is 18 hours of light and 6 hours of darkness, with a PPFD of 600. We raised the nutrient concentration by 100 ppm, increased the light intensity by 100 PPFD, and lowered the humidity to 65%. The plants have grown significantly, primarily stretching upward with their main colas and side branches. Visually, there are no signs of nutrient deficiencies or toxicities; the plants are absorbing their 650 ppm well, with no salt buildup or burn.

Day 79, 28th of November 2020: Hi there! Here we go let's say half way. Plants are pretty these Original Sensible Seeds genetics are fantastic! The 2 OG and the Runtz Gum is very strechy. Black Ghost is pretty shorter bushier plant. Runtz Gum and Do-Si-Dos OG approx the same height Do-Si-Dos OG is taller a bit. Wedding Gelato is really cool little, bushy anf nice buds forming not problem with her all she is quiet. Do-Si-Dos OG has some deficiency and leaf gets dry but nothing very serious..... Fertilization is still the same every second day with the rationand mixture above stated. The lamp is on 11.15 min and off 12.45 min. Last week was 15 min longer light cycle.... So every week 15 min shorter light cycle until the 5th week. So far -45 min. It switches on at 6 am and off at 17.15 pm.