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@Hydronaut
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Big healthy plant for the most part, but the flowering just won’t kick in! This is the week that says she’s running behind or that she’s going to be on a cocaine diet till the end? Need some bulk, it’s week 8 already. I don’t like my girls fast anyways, so I’ll be a patient gent. Day 51: Early AM: It seems that the flowers are starting to fatten up at a familiar rate now. Stretch is definitely over to boot. Feels like a week or so behind my other Auto grows, but I am gathering from the peeps on GD that grow times can be all over the place with Autos; even with seeds front the same plant. I won't be a Debbie-downer, but I cant help but think what this thing would have done in a bigger space. I left her alone a couple days ago as her stretch slowed noticeably. A pluck here and there moving forward, but that's about it for the sadistic stuff. Day 51: Late Afternoon: She's about to take off in the flower it feels. 6 hours passed, and I was like "oh, hello there!" If nothing goes too crazy, I'm hoping she'll be a good one regardless of her 3.8 sf living quarters. I never did move the top shelf, so the light is less than 8 inches from the canopy. I'll keep an eye on the "crispy effect" out of complete laziness and fear of dropping the light on the plant! Tallest branches are around the perimeter and still hitting their DLI. The center/down the plant is getting blasted by the light, so I suspect about 6 or so runners to hit the canopy and hang out with the FIM family in the next week or so? Day 51: Evening: Bigger bucket = bigger plant in hydro? One heck of a root mass going on in her trough. I tried pushing the roots to the back of the bucket so I could ph without getting roots directly. Nope! That bucket is filled with roots, and its an 8 gallon bucket. I would have never thought an auto would have enough time to grow into an 8 gallon bucket. I was banking on some extra nutrient volume, so I could go out of town for 4-5 days. Hard to say on bucket size though, I have grown 10 foot tomato monsters in 3 gallon Ebb/Flow pots so maybe she would have grown the same in a 5 gallon bucket? I don't think that much root volume could grown in an 5 gallon bucket, so bigger bucket = bigger plant here? Things that make you go "hmmm..." Day 53: A lot of undesirable undergrowth that blew up during stretch. Like a ton of it, but I’m done messing with her. Took out the top shelf and reconfigured exhaust. What a pain. I didn’t drop the light but the IP cam took a nose dive on a branch. Now the light is about 12-14” from canary instead of 6-8'. She started to get some light burn so I had to do the deed. Wider IP camera view with added distance so that's a plus! Plant is very happy at 550-575ppm. 600ppm seems to be the number without burning plants? Humidity, temp, and ppm AND light distance in check. Raising the light opened the focal point of this light quite a bit; especially in the center where the PPFD was over 2500 at the center and light less than 8 inches from the canopy. Feeling good. All is in check with some room to grow. She will gain another 2" in height I presume through flower. Day 54: The jury is out of this light. I hits my dli from veg to harvest with it's fixed position design, but wondering if 50watts more would have been the number. I'm going to keep the light in there to keep the data flow congruent to its original design. I hit my DLI right now from the top of the canopy to about 10-12" down the plant. Lighter specs from my last grow, but its hard to tell what is getting what once you leave top of the canopy. Hopefully it will suffice! I moved the light a few inches closer as my DLI was getting light in some of the corners/perimeter. She right 10-11" and is about as good as she is going to get given150watts. I am realizing that 200 watt is probably the optimized wattage here if some canopy penetration is desired, but light these dimensions are not plentiful[. 250 watt and that would be max with managing heat and exhaust sound levels. 250 watt would accommodate a fix state design no problem. I hate to change the light variable but I only have the luxury of one-at-a-time grows, so everything counts on some levels. Been using the PAR meter on and off throughout the day. Penetration needs to be about 25% stronger. Its a perfect light for your average auto grow, but if these results repeat themselves a 250 watt is probably in order. I am making the executive decision to install a 250 watt bar light. I found one with a small footprint at 240watts. Hard find, specs are more than adequate for 4 sf. Will maybe get down a bit deeper but things are looking really crammed with the bottom half. It is PACKED with bud sites and likely not enough light. Questioning the light change, but will definitly thin some lower stuff and "officially" let her be. Since in bumped up the CalMag to about 1.5 ml/gal the reservoir has been running ph equilibrium for 6 days now at 600ppm. I’m guessing this deficiency was driving down ph instead of the usual up/equilibrium in week 7. My first auto to eat at 600ppm for a week with no tip burn. She looks fit, I’ll keep her diet here as the max. I have never cut MicroGro out completely in a grow before. I have been faithful to GH 7 part series since for long time. This grow I took the GH feeding chart and worked backwards to a ppm level I found on the web to be an acceptable range for Autos. These ratios have traditionally been acceptable and without issue. Beautiful plants every time. On the last two runs with this concocted feeding schedule Cal/Magic was very light until week 7, and exactly when I started to see rust spots, etc. in this grow. After a 2ml/gal dose of CaliMagic plus addbacks on watering, that issue disappeared overnight. I mean zero issues with any new or progressing growth. Here's the take away with this. Although CalMag issues are easy to spot and resolve, I did learn is that in both runs they HAD A PERSISTANT DROP IN PH IN MY RESERVOIR in late veg/tranistion. Another wards, if you PH is dropping and your ppm are not doing anything, it very well could be a CalMag deficiency. An indication know before the leaves start getting spotted, etc. I guess if one were perceptive enough, you could resolve this issue as it happens, and never see the effect on the leaves? On this current run, as soon as I dosed the bucket good with Cal/Mag and kept my add/backs a 1.5/ml/gal the PH locked in big. Going on 7 days at 600ppm, 6.0 and the ph is steadily rising. Maybe another bump in ppm. No sign of tip burn yet, why the heck not!:) Not really the expected progress this week but she may just need time. I don’t think she’ll really bulk up until the end of week 9 / week 10. New light to arrive Tuesday but not sure a stronger light will get much further down. It’s almost a roadblock to get beyond the main tops and they are getting well more than they can handle light wise. Bigger light may only mean more heat.
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@starke
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Day 71 - 27.1.20 noticed swelling of calyx. checking trichomes everyday. Day 72 - 28.1.20 watered 3l with 2ml bloom, 2ml bud candy and 2ml flawless finish. Day 73 - 29.1.20 checking trichomes, sugar leaver a good 30% amber, calyx trichomes all milky. Day 74 - not sure when to harvest or prep them for harvest Day 76 - shes getting more into fall, some more leaves yellow-pink; more swelling but also some new white pistols :) she's a sweetheart.
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Purtroppo la auto elephant mi ha abbandonato.. Peccato! Metterò subito un altro seme a germogliare anche se già sono passate 2 settimane e mezzo, non fa niente, sono fiducioso!
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There we go, it‘s week #10 with Skunk #1 and these beautiful pictures. The EC is at 2.0 and the ph at 5.8... let’s see them still growing in the last 2 weeks. Njoy! Day 64: New pictures online! EC is going up every day just like ph. Problem today is the heat! The water in the tank has exceeded 25°C and the temperature is not going to be cooler in the next week. That is why I added Purolyt. To prevent the water in the tank from getting algae and other sh*t. 😎 Day 65: Maybe it was too much Purolyt yesterday or the plants just give away all the leafes that are not in the light. Have to check tomorrow with lights off if something went wrong. Put 10liter pure water into the tank and ph'ed down. Day 66: I know the water in the tank is too warm with 26° C. I will have to solve this problem. (I think I will use icecubes or something like that) But the flowers look pretty good. They stand the heat better than we do.(35° C outside in the city!) Day 67: Still problems with the water temperature in the tank. Put an ice pack inside to get the temp down. Won't be helping long but for a moment. Day 69: Still problems with the hot summer. Heat is up to 31,2° C but the plants seem to like it. Water temp went down to 24.4° C after refilling 10l pure water. After that I checked the EC which is at 1.86 and ph'ed down to 5.6 Planning to harvest soon, maybe in one week, what do you guys think? The white spots in the blossoms are burned blossoms that contains no thc or cbd. It looks like an albino flower to me. 😌 I can't avoid them because the tent is too low.
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@Snakeking
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Everything is fine i still water-and feed them 5 times a week 2 times light compost tea with bath guano and molasses. I startd to add iguana juice bloom in my compost tea! Lets see the reaction of my girls. I also add a little bit of rhino skin by advanced nutrients against heat wave
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I'm so fuckin' crazy, i change my hps lamp 250w for a led lamp 600w 👽⛈️
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@Bmy88
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Crecen muy rápido, esta semana apliqué top veg, y empezaré con microbita dentro de unos días.
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@nonick123
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Día 13 (06/05) Nos acercamos al final de la fase plántula. A ver si ahora empiezan a crecer de forma explosiva! He sido muy estricto con los riegos, dejando que el top hasta el nudillo se seque completamente! Día 14 (07/05) Hago una mejora en la tienda. Hasta ahora tenia intracción pasiva, pero ahora he instalado una turbina para que impulse aire fresco dentro de la tienda Día 15 (08/05) Con el calor que hace me sorprende que las macetas sigan aguantando la humedad, pero al hundir el nudillo sigue húmeda. Mañana haré un riego de nuevo Día 16 (09/05) Relleno el top con sustrato para compensar la compactación y dar mas soporte a las plantas Riego con 300 ml solo H20 - pH 6,2 Día 17 (10/05) Baño de sol de dos horas. Día 18 (11/05) Baño de sol de dos horas Día 19 (12/05) Riego 750 ml H2O + Regulator 0,15 ml/l + CaMg-Boost 0,25 ml/l + Startbooster 0,25 ml/l - pH 6.2 💦Nutrients by Aptus Holland - www.aptus-holland.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae
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@w00tGrow
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Plant is looking good, budds are getting bigger and give sweet sweet aroma. I have not changed much for last 3 weeks, defolation here there only.
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@SAC87
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Day 70 Well things have really turned around this last week. The buds are hard. They have gotten significantly firmer since flushing. The smell is super potent of tropical, sweet citrus. It and the others are too much for my old carbon filter to handle. I have another ordered. So now I’m just watering when dry til the trichomes are ready and watching the colors change!!! Happy Growing 🌱👍🏻
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@CalGonJim
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9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!
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(translate.google). Bad bloody will he become tall and handsome? to be continued ;) 21/03/2020 First watering with fertilizer BloodySkunk and the largest closely followed by the Blackberry I am sure their baby will be very beautiful
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@Theia
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I love this plant. She is so strong and grows with a lot of vigor. I am a little annoyed as I have been quite busy so I have been feeding the room the same mix and I think this one is started to show signs she is pissed off at me . There are the start of nutrient burn on her tips. I do not like this. I do not want this. So I I'll make her a slightly different feed this week and reduce the EC by a bit. Back down to a 1.5-1.6ec . Lights are at 40% drawing 180w. Lovely..😎 Uvb is not on yet as I have been away working and don't want to scare my wife away from the great job she does feeding when I'm away.. so I will deploy it as soon as I'm back and can be around to observe the effect.. it's all new to me so a bit of a lesson to carry forward to future grows... Thanks for passing. Grow well 🌿💚🌿🙏🏼
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@nonick123
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Día 76 (08/07) Riego con 1 Litro H2O + Regulator 0,15 ml/l + K-Boost 1 ml/l + CaMg-Boost 0,3 ml/I + TopBooster 0,3 ml/l - pH 6.2 Ultimo riego con nutrientes. Empezamos el flush (aunque no soy muy partidario del flush, voy a probar las indicaciones de Aptus) Día 77 (09/07) La planta empieza a llegar al final de ciclo, porque ha reducido el consumo de agua en los últimos días Riego con 750 ml sólo H20 - ph 6.2 Día 78 (10/07) Riego con 750 ml sólo H20 - ph 6.2 La senescencia está llegando. Efectivamente solo le faltan unos días para la cosecha! 😍💥💨😁 Día 79 (11/07) Riego con 750 ml sólo H20 - ph 6.2 Revisión con el microscopio 60x! Los tricomas están 80% trasparentes + 20% de turbios. La cosecha se va a demorar unos 4-5 días más Día 80 (12/07) Riego con 750 ml sólo H20 - ph 6.2 He sufrido un ataque de gusanos y tengo que eliminar tres cogollos... Los últimos días no he podido estar encima de ella tanto como me gustaría... 😞 Día 81 (13/07) Riego con 750 ml sólo H20 - ph 6.2 Vuelvo a revisar los tricomas y sigue sin estar lista.... Status tricomas: 50% turbios + 50% trasparentes Día 82 (14/07) Hace mucho calor aqui! 37 ºC Riego con 1000 ml sólo H20 - ph 6.2 💦Nutrients by Aptus Holland - www.aptus-holland.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae
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Over watered the wedding cake so I transplanted her into dry soil with food already in it but the food was 2 strong and burnt her so I quickly cut as many clones as I could (6) and trashed the rest of the plant so this grow is done but gonna replace this grow with a girl scout cookies got 2 of them so that's what's in the video there in fox farm ocean forrest soil they have not been fed yet growing kinda slow but looking very nice
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@Canna96
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Hey now, this week went pretty good, I am still running a 19/5 light schedule, and feeding her every 6 hours. She actually grew about 7 inches this week, so she is now at 16 inches. I am thinking between 1 and 2 more weeks of Veg, then I will switch her to 12/12. I would like to get her to about 24 inches tall when I flip her. I made another quick time lapse this week, if anyone has any suggestions for songs hit me up. The canna sugar still has the tincture in it, but it is dissolving and should be ready later today. I hope everyone had a great week, stay safe and Blaze On!